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The alpha-amino nitrogen of the glycine, the deprotonated amide nitrogen of the peptide bond between glycine and histidine, and an imidazole nitrogen of the histidine side chain form the primary coordination sphere

This increase was corroborated by elevated expression of aconitase and activity of succinate dehydrogenase (Krebs cycle enzymes), as well as increased activity of NADH dehydrogenase and expression of H-ATPase (oxidative phosphorylation enzymes)

If initial staining was insufficient, the sample was sectioned again to obtain a better visual field of cartilage structure

Toxicology International, 24(1), 65-73

Furthermore, researchers who buy cerebrolysin alongside these complementary compounds from a single source eliminate batch-to-batch variability as a confounding factor